Strain Information | |
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Image | |
BRC No. | RBRC06468 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6J-Ppp1r17<tm1Nkz> |
Former Common name | G-substrate KO, G-substrate KO |
H-2 Haplotype | |
ES Cell line | MS12 [C57BL/6] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Shogo Endo, Tokyo Metropolitan Institute of Gerontology. MS12 ES cells derived from C57BL/6 were used to generate the mutant mice. C57BL/6J background. |
Strain description | G-substrate (Ppp1r17) gene knockout mice. Exon 2 was replaced with a tau-AFAP-PGK-neo cassette. The PGK-neo was subsequently removed via CAG-cre mediated recombination. Homozygous mutant mice exhibit loss of long-term OKR adaptation and temporary loss of cerebellar LTD in early developmental stage. No obvious ataxia or abnormal movement. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Proc. Natl. Acad. Sci. USA, 106, 3525-3530 (2009). 19218432 Mol. Brain Res., 35(1-2) 58-68 (2005). 15857669 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ppp1r17protein phosphatase 1, regulatory subunit 176Ppp1r17<tm1Nkz>targeted mutation 1, Toru Nakazawa Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter AFAPGreen Fluorescent Protein (Aequorea victoria)6AFAP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter taubovine tau protein cDNA6tau |
Ordering Information | |
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Donor DNA | bovine tau cDNA, jellyfish GFP derivative (AFAP) cDNA, phage P1 loxP site, mouse Gsbs (Ppp1r17) genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A, 106, 3525-3530 (2009).Required the closing of MTA with Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology. |
Depositor | Shogo Endo (Tokyo Metropolitan Institute of Gerontology) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |