Strain Data Sheet

RBRC06468

Strain Information

Image
BRC No.RBRC06468
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ppp1r17<tm1Nkz>
Former Common nameG-substrate KO, G-substrate KO
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shogo Endo, Tokyo Metropolitan Institute of Gerontology. MS12 ES cells derived from C57BL/6 were used to generate the mutant mice. C57BL/6J background.
Strain descriptionG-substrate (Ppp1r17) gene knockout mice. Exon 2 was replaced with a tau-AFAP-PGK-neo cassette. The PGK-neo was subsequently removed via CAG-cre mediated recombination. Homozygous mutant mice exhibit loss of long-term OKR adaptation and temporary loss of cerebellar LTD in early developmental stage. No obvious ataxia or abnormal movement.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 106, 3525-3530 (2009). 19218432
Mol. Brain Res., 35(1-2) 58-68 (2005). 15857669

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ppp1r17protein phosphatase 1, regulatory subunit 176Ppp1r17<tm1Nkz>targeted mutation 1, Toru Nakazawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
AFAPGreen Fluorescent Protein (Aequorea victoria)6AFAP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
taubovine tau protein cDNA6tau

Ordering Information

Donor DNAbovine tau cDNA, jellyfish GFP derivative (AFAP) cDNA, phage P1 loxP site, mouse Gsbs (Ppp1r17) genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A, 106, 3525-3530 (2009).Required the closing of MTA with Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology.
DepositorShogo Endo (Tokyo Metropolitan Institute of Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data