Strain Data Sheet

RBRC06476

Strain Information

Image
BRC No.RBRC06476
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Adam18<tm2Osb>/2
Former Common nameAdam18#2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2012. EGR-G101 ES cells derived from a Tg mouse in C57BL/6N background were used. C57BL/6N background.
Strain descriptionAdam18 knockout mice.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688

Health Report

Examination Date / Room / Rack

Gene

Gene info

Ordering Information

Donor DNAchicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse Adam18 genomic DNA, SV40 polyadenylation site, mouse PGK promoter, E.coli. Neomysin phosphotransferase gene (neoR), Yeast FRT site, Phage P1 loxP site
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data