Strain Data Sheet
RBRC06493
Strain Information | |
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| Image | |
| BRC No. | RBRC06493 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | ICR.Cg-Hes1<tm1(venus)Imayo> |
| Former Common name | Venus-Hes1 fusion KI (Neo), Hes1/venus KI |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2008. TT2 ES cells were used. The mutant mice were crossed to ICR. |
| Strain description | Venus/Hes1 knock-in mice. Venus-Hes1 fusion protein is expressed from Hes1 locus. LoxP sequences were knocked into the first intron and downstream of 3'UTR of Hes1 gene for conditional knock-out study. delta Neo type (RBRC06010), fNeo type (RBRC06493). |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to Slc:ICR] |
| References | Science, 342(6163):1203-8. doi: 10.1126/science.1242366. 24179156 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hes1hairy and enhancer of split 1 (Drosophila)16Hes1<tm1(venus)Imayo>targeted mutation 1, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)16Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)16mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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| Donor DNA | Jellyfish venus cDNA, phage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Hes1 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 342(6163):1203-8. doi: 10.1126/science.1242366.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |