Strain Data Sheet
RBRC06513
Strain Information | |
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| Image | |
| BRC No. | RBRC06513 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Pax6<tm2(iCre)Fmur> |
| Former Common name | Pax6F2AiCre, B6-Pax6/F2Acre KI |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | C57BL/6NCrl |
| Appearance | |
| Strain development | Developed by Fujio Murakami, Graduate School of Frontier Biosciences, Osaka University in 2014. RENKA ES cells were used to generate the mutant mice. |
| Strain description | Pax6 hgene Cre knock-in mice. F2A, codon improved Cre-polyA and frt-flanked pgk/gb2-neo cassettes were inserted into the exon 13. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrlCrlj] |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter F2Afoot-and-mouth disease virus (FMD) 2A peptide2F2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter iCrephage P1 codon improved Cre (iCre)2iCre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal2 |
Ordering Information | |
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| Donor DNA | Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Bacteriophage P1 codon improved Cre (iCre), SV40 PolyA signal, Saccharomyces cerevisiae Flp recombinase target (FRT) sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Pax6 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Neurobiology Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Fujio Murakami, Osaka University) and DEVELOPER (Dr. Kenji Sakimura, Nigata University) is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Fujio Murakami (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |