Strain Information | |
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Image | |
BRC No. | RBRC06514 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | STOCK Dock5<Gt(AN0268)Wtsi>/Shigm |
Former Common name | Dock5 KO, Dock5 Gt |
H-2 Haplotype | |
ES Cell line | E14TG2aIV [129P2/OlaHsd] |
Background strain | |
Appearance | agouti |
Strain development | Developed by Jean-Franc Francois Cote, Montreal University. Maintained by Hiroshi Hiai, Shiga Medical Center for Adults. 129, BALB/c and other strain? mixed background. |
Strain description | Dock5 gene trap mice. The pGTOlxr gene trap vector containing a beta-geo cassette was inserted into the intron 29 of Dock5 gene. |
Colony maintenance | Homozygote x Homozygote |
References | Proc. Natl. Acad. Sci. USA, 105. 15446-15451 (2008). 18820033 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dock5Dedicator of cytokinesis 514Dock5<Gt(AN0268)Wtsi>gene trap AN0268, Wellcome Trust Sanger Institute Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)14 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence14lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)14 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA14 |
Ordering Information | |
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Donor DNA | pGT0Lxr gene trap cassette (1.5 kb of mouse En2 intron 1, mouse En2 splice acceptor, yeast FRT (flipase recombination target) sites, phage P1 lox71 site, phage P1 loxP site, E.coli lacZ, E.coli neo, SV40 polyA, pUC backbone) |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 15446-15451 (2008).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Kazuo Kinoshita (Shiga Medical Center Research Institute) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |