Strain Information | |
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Image | |
BRC No. | RBRC06517 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Gt(ROSA)26Sor<tm10(PITT)Maoh> |
Former Common name | TOKMO-3, B6-ROSA26/PITT KI |
H-2 Haplotype | |
ES Cell line | EGR-101 [C57BL/6N] |
Background strain | |
Appearance | |
Strain development | Developed by Masato Ohtsuka, Tokai University School of Medicine in 2014. EGR101 ES cells were used to generate the mutant mice. |
Strain description | SA-F14-attP-JT15-eGFP-T2A-neo-hOCT4 ZFN target site-pA-FRT-L-lox2272-F15 ROSA26 knock-in mice. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6N] |
References | BMC Genomics 2015, 16:274 25887549 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm10(PITT)Maoh>targeted mutation 10, Masato Ohtsuka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter F14yeast FRT (flippase recombination target) site (synthetic) variant (F14)6F14 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter F15yeast FRT (flippase recombination target) site (synthetic) variant (F15)6F15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FRT-Lyeast FRT (flippase recombination target) site, variant (FRT-L)6FRT-L Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter JT15phage P1 loxP variant (JT15)6JT15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)6T2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter attattachment site6att Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter attattachment site6att Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox2272phage P1 loxP variant6lox2272 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal6 |
Ordering Information | |
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Donor DNA | mouse Gt(ROSA)26Sor, adenovirus SA, yeast FRT variant (F14), Streptomyces lividans and actinophage phi C31 attP, phage P1 loxP variant (JT15), jellyfish eGFP cDNA, insect Thosea asigna virus T2A peptide, E. coli neo, human OCT4 cDNA, Simian virus 40 (SV40) polyA, yeast FRT variant (FRT-L), phage P1 loxP variant (lox2272), yeast FRT variant (F15) |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in the first publication of the results obtained from the use of the BIOLOGICAL RESOURCE. |
Depositor | Masato Ohtsuka (Tokai University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Mouse of the Month Sep 2015 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |