Strain Data Sheet

RBRC06545

Strain Information

Image
BRC No.RBRC06545
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prickle1<tm1.1Hmd>
Former Common namePrickle1 flox, B6-Prickle1 floxed
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Masakazu Hashimoto, Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain descriptionPrickle1 gene floxed mice. Exon 6 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesDev. Cell., 40(5):439-452.e4(2017). 28292423

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prickle1prickle homolog 1 (Drosophila)15Prickle1<tm1.1Hmd>targeted mutation 1.1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Prickle1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 40(5):439-452.e4(2017).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data