Strain Data Sheet

RBRC06546

Strain Information

Image
BRC No.RBRC06546
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prickle1<tm1.2Hmd>
Former Common namePrickle1 delta ex6, B6-Prickle1 KO
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Masakazu Hashimoto, Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. Flanked Exon 6 was removed by crossing with CAG-Cre mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain descriptionExon 6 of Prickle1 gene was removed.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesDev. Cell., 40(5):439-452.e4(2017). 28292423

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prickle1prickle homolog 1 (Drosophila)15Prickle1<tm1.2Hmd>targeted mutation 1.2, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Ordering Information

Donor DNAphage P1 loxP site, mouse Prickle1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 40(5):439-452.e4(2017).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data