Strain Information | |
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Image | |
BRC No. | RBRC06547 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Prickle2<tm1Hmd> |
Former Common name | Prickle2 flox(neo)#60, B6-Prickle2/fneo |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Masakazu Hashimoto, Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. C57BL/6 and 129 mixed background. |
Strain description | Prickle1 gene floxed neo mice. Exon 6 was flanked by loxP sites. A frt flanked PGK-neo cassette was inserted after the exon 6. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Dev. Cell., 40(5):439-452.e4(2017). 28292423 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter polyA6 |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), mouse polyA, E. coli neo, mouse Prickle2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 40(5):439-452.e4(2017). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |