Strain Data Sheet

RBRC06550

Strain Information

Image
BRC No.RBRC06550
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nodal<tm3.2Hmd>
Former Common nameNodalΔRAREn, B6-Nodal/RAREn KO
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Masayuki Uehara and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. Flanked REREn was removed by crossing with CAG-Cre mice (B6/129 background). A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain descriptionREREn in intron 1 of the Nodal gene was removed. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesGenes Dev., 23, 1689-1698 (2009). 19605690

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nodalnodal10Nodal<tm3.2Hmd>targeted mutation 3.2, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Ordering Information

Donor DNAphage P1 loxP site, yeast FRT (flipase recombination target) site, mouse Nodal genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 23, 1689-1698 (2009).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data