Strain Data Sheet

RBRC06574

Strain Information

Image
BRC No.RBRC06574
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Chx10-cre/ERT2)G7Tfur
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2010. The BAC-Chx10-creERT2 transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain descriptionBAC-Chx10-creERT2 transgenic mice. This transgenic mouse expresses tamoxifen-inducible cre recombinase under the control of the mouse Chx10 promoter. 129 background (RBRC05565), C57BL/6 mixed background (RBRC06574).
Colony maintenanceCarrier x Carrier, Noncarrier [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse Vsx2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

Donor DNAMouse Vsx2 genomic DNA, P1 phage Cre recombinase, Human Mutated Estrogen receptor LBD, SV40 polyA signal, Phage P1 loxP derived from pBAC vector
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data