Strain Data Sheet

RBRC06800

Strain Information

Image
BRC No.RBRC06800
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ncaph<tm1.1Hiran>
Former Common nameNcaph floxed, B6-Ncaph floxed
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Kenji Nishide and Tatsuya Hirano, Chromosome Dynamics Laboratory, RIKEN. BA1 ES cells (C57BL/6 x 129/SvEv) were targeted by using standard conditional knockout methods by inGenious Targeting Laboratory (NY, USA). The mice were mated with CAG-FLPe transgenic mice to delete the Neo selection cassettes, and subsequently backcrossed to C57BL/6J over nine generations.
Strain descriptionNcaph gene floxed mice. Condensins I and II are evolutionarily conserved protein complexes and thought to be central to chromosome assembly and segregation among eukaryotes. In Ncaph floxed mice, exons 2-4 of the Ncaph gene (encoding the condensin I-specific subunit CAP-H) are flanked by loxP sites. Tissue-specific deletion of Ncaph can be generated by crossing with various Cre-expressing mice to study condensin I-specific functions. Homozygous floxed mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
ReferencesPLoS Genet. , 10(12):e1004847. (2014). 25474630

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ncaphnon-SMC condensin I complex, subunit H2Ncaph<tm1.1Hiran>targeted mutation 1.1, Tatsuya Hirano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ncaph genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet. , 4;10(12):e1004847. (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorTatsuya Hirano (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jun 2016
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data