Strain Information | |
---|---|
Image | |
BRC No. | RBRC06801 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ncaph2<tm1.1Hiran> |
Former Common name | Ncaph2 floxed, B6-Ncaph2 floxed |
H-2 Haplotype | |
ES Cell line | iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Nishide and Tatsuya Hirano, Chromosome Dynamics Laboratory, RIKEN. BA1 ES cells (C57BL/6 x 129/SvEv) were targeted by using standard conditional knockout methods by inGenious Targeting Laboratory (NY, USA). The mice were mated with CAG-FLPe transgenic mice to delete the Neo selection cassettes, and subsequently backcrossed to C57BL/6J over seven generations. |
Strain description | Ncaph gene floxed mice. Condensins I and II are evolutionarily conserved protein complexes and thought to be central to chromosome assembly and segregation among eukaryotes. In Ncaph2 floxed mice, exons 3-8 of the Ncaph2 gene (encoding the condensin II-specific subunit CAP-H2) are flanked by loxP sites. Tissue-specific deletion of Ncaph2 can be generated by crossing with various Cre-expressing mice to study condensin II-specific functions. Homozygous floxed mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] |
References | PLoS Genet. , 4;10(12):e1004847. (2014). 25474630 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ncaph2non-SMC condensin II complex, subunit H215Ncaph2<tm1.1Hiran>targeted mutation 1.1, Tatsuya Hirano Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP |
Ordering Information | |
---|---|
Donor DNA | phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ncaph2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet. , 4;10(12):e1004847. (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Tatsuya Hirano (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Mouse of the Month Jun 2016 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |