Strain Information | |
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Image | |
BRC No. | RBRC06802 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Smc2<tm1.1Hiran> |
Former Common name | Smc2 floxed, B6-Smc2 floxed |
H-2 Haplotype | |
ES Cell line | iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Nishide and Tatsuya Hirano, Chromosome Dynamics Laboratory, RIKEN. BA1 ES cells (C57BL/6 x 129/SvEv) were targeted by using standard conditional knockout methods by inGenious Targeting Laboratory (NY, USA). The mice were mated with CAG-FLPe transgenic mice to delete the Neo selection cassettes, and subsequently backcrossed to C57BL/6J over eight generations. |
Strain description | Smc2 gene floxed mice. Condensins I and II are evolutionarily conserved protein complexes and thought to be central to chromosome assembly and segregation among eukaryotes. In Smc2 floxed mice, exons 10-12 of the Smc2 gene (encoding a shared subunit of both condensins) are flanked by loxP sites. Tissue-specific deletion of Smc2 can be generated by crossing with various Cre-expressing mice to study the functions of both condensins. Homozygous floxed mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] |
References | PLoS Genet. , 4;10(12):e1004847. (2014). 25474630 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Smc2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet. , 4;10(12):e1004847. (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Tatsuya Hirano (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |