Strain Information | |
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Image | |
BRC No. | RBRC06805 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Gt(ROSA)26Sor<tm1(dach1)Hhsk> |
Former Common name | Dach Tg, B6-ROSA26/Dach1 KI |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Haruko Hayasaka, Graduate School of Medicine, Osaka University. RENKA ES cells were used to generate the mutant mice. |
Strain description | Dach1 ROSA26 conditional knock-in mice. Targeted insertion of SA-loxP-CAT-PGK-neo-loxP-Dach1-pA cassette into the mouse Rosa26 locus. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(dach1)Hhsk>targeted mutation 1, Haruko Hayasaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dach1dachshund 1 (Drosophila)6Dach1<tm1Hhsk>targeted mutation 1, Haruko Hayasaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal6 |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, E. coli cat, bovine growth hormone polyA signal, mouse Dachshund1 cDNA, adenovirus type2 Splicing acceptor (SA), yeast FRT (flipase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 polyA signal, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cancer Research Cre/loxP system Developmental Biology Research FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT, which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not -for-profit research, is required to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Haruko Hayasaka (Kindai University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |