Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC09240  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name STOCK Zeb2<tm2.1Yhi>  
Former Common name SIP1-EGFP, Zfhx1b/GFP KI, STOCK Zfhx1b<tm2.1Yhi>  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yujiro Higashi, Aichi Human Service Center in 2008. R1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice (B6). ICR mixed background (RBRC09239), ICR and C57BL/6 mixed background (RBRC09240).  
Strain description SIP1 (Zeb2, Zfhx1b) gene GFP knock-in mice. A EGFP was inserted in-frame with the coding sequence in exon 9 of the Zeb2 gene.  
Colony maintenance Heterozygote x Wild-type  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 2 Symbol Zeb2  
Symbol name zinc finger E-box binding homeobox 2  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 2  
Common name No Data  
Symbol description No Data  
Research applications Cre/loxP system,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Higashi, Yujiro (AICHI HUMAN SERVICE CENTER)  Higashi, Yujiro 
Strain Status /
(Expected delivery)

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