Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC09257  
Type TransgeneCartagena  
Species Mus musculus  
Strain name C.Cg-Tg(CAG-tdKaede)15Utr  
Former Common name Kaede (WK-m15), BALB-CAG-Kaede Tg#15  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Michio Tomura, Kyoto University Graduate School of Medicine and Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Atsushi Miyawaki, RIKEN Brain Science Institute. The Tg mice were backcrossed to BALB/c over 22 times.  
Strain description These transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light.  
Colony maintenance Carrier x Noncarrier [BALB/cAJcl]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name SV40 PolyA site  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 2 Symbol tdKaede  
Symbol name Trachyphyllia geoffroy Kaede cDNA  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc Natl Acad Sci U S A. 105, 10871-10876 (2008).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Kaede using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR Dr. Yoshihiro Miwa (ymiwa@md.tsukuba.ac.jp) of University of Tsukuba and obtain a use permission.  
Additional information
 1 Necessary documents for ordering: 1. Approval form (form D) from the depositor (ENG) , (JPN) , 2. Approval form (form V) from the developer, 3. MTA for distribution with RIKEN BRC and 4. Order form (ENG) , (JPN) .  
 2 Genotyping protocol <PCR>  
Depositor Miwa, Yoshihiro (Graduate School of Comprehensive Human Sciences, University of Tsukuba)  Miwa, Yoshihiro 
Strain Status /
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