Strain Data Sheet

RBRC09390

Strain Information

Image
BRC No.RBRC09390
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Nkapl<tm1(KOMP)Vlcg>/1Htan
Former Common nameNkapl KO
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesPLoS One 10, e0124293 (2015). 25875095

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
NkaplNFKB activating protein-like13Nkapl<tm1(KOMP)Vlcg>targeted mutation 1, Velocigene

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13human Ubiquitin C promoter

Ordering Information

Donor DNAVelocigene cassette ZEN-Ub1(E. coli LacZ-p(A), Phage P1 loxP sites, human Ubiquitin C promoter, E. coli Neomycin resistance gene, polyA), mouse Nkapl genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The mice were originally created by the National Institutes of Health (NIH) Knock-Out Mouse Program (KOMP). The RECIPIENT is free to publish, present, display, disclose or seek patent or other intellectual property protection on any inventions arising from the use of the KOMP Materials, with no restrictions or obligations of any kind, so long as the RECIPIENT uses reasonable efforts to credit or otherwise acknowledge the KOMP as the creator of the KOMP Materials.
DepositorHiromitsu Tanaka (Nagasaki International University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data