Strain Data Sheet

RBRC09393

Strain Information

Image
BRC No.RBRC09393
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Kcnj11<tm2Sse>
Former Common nameKir6.2-flox
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Susumu Seino, Kobe University Graduate School of Medicine. ES cells derived from C57BL/6 were used. C57BL/6 genetic background.
Strain description
Colony maintenance
ReferencesJ. Clin. Invest., 130(12):6639-6655 (2020). 33196462

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kcnj11potassium inwardly rectifying channel, subfamily J, member 117Kcnj11<tm2Sse>targeted mutation 2, Susumu SeinoKir6.2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)7GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAPhage P1 loxP sites, Yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Bovine Growth hormone poly A signal, mouse Kcnj11 genomic DNA
Research applicationCre/loxP system
Endocrine Deficiency Research
FLP/frt system
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorSusumu Seino (Kobe University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data