Strain Data Sheet
RBRC09601
Strain Information | |
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| Image | |
| BRC No. | RBRC09601 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Ccdc86<tm1Hfuj> |
| Former Common name | Cyclon-KO(EN) |
| H-2 Haplotype | |
| ES Cell line | iTL IC1 [C57BL/6NTac] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Hodaka Fujii, New York University in 2009. iTL IC1 ES cell line [C57BL/6NTac] was used. C57BL/6 background. |
| Strain description | Ccdc86 (coiled-coil domain containing 86, Cyclon) knockout mice. EGFP and pgk-neo cassettes were placed just downstream of translation initiation codon in exon 1 of the gene. Heterozygous mice exhibited reduced Fas expression in activated T lyphocytes. Homozygous mutants are emobryonic lethal. |
| Colony maintenance | |
| References | Blood, 13;114(7), 1355-1365 (2009). 19528538 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ccdc86coiled-coil domain containing 8619Ccdc86<tm1Hfuj>targeted mutation 1, Hodaka Fujii4933411H20Rik, 6720480F16Rik, cyclon, D19Ertd678e Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)19EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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| Donor DNA | P1ファージ loxP, オワンクラゲ Green Fluorescent Protein (GFP)(緑色蛍光蛋白質), マウス Cyclon蛋白質遺伝子, マウス phosphoglycerate-kinase 1 (pgk) プロモーター, 大腸菌 ネオマイシン耐性遺伝子 |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon. Saint Fleur S, Hoshino A, Kondo K, Egawa T, Fujii H. Blood (2009) 114 (7): 1355-65. The BIOLOGICAL RESOURCE shall be used only for academic research purposes by not-for-profit institutions. Use of the BIOLOGICAL RESOURCE by for-profit institutions or use for commercial research purposes shall require a commercial license from the Depositor. A recipient shall also acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of their use of the BIOLOGICAL RESOURCE. |
| Depositor | Hodaka Fujii (New York University School of Medicine) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |