Strain Information | |
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Image | |
BRC No. | RBRC09607 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6J-Avp<tm1(Eluc)>Chron |
Former Common name | AVP-ELuc |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Kenichi Honma and Sato Honma, Graduate School of Medicine, Hokkaido University (2015). C57BL/6J background. |
Strain description | Knock-in mice of emerald-luciferase (Eluc) in the Arginine vasopressin (AVP) gene locus. AVP is expressed at the paraventricular hypothalamic nucleus, supraoptic nucleus and suprachiasmatic nucleus. Homozygotes die by postnadal day 10. A DNA construct of ELuc-Neo was knocked into the regions upstream of the translation initiation site of the mouse AVP locus. |
Colony maintenance | |
References | Eur. J. Neurosci., 42, 2678-2689 (2015). 26342201 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ELucEmerald luc luciferase (Pyrearinus termitilluminans)2ELuc Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter |
Ordering Information | |
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Donor DNA | beetle Emerald-Luciferase cDNA, mouse PEST sequence(ornithine dehydrogenase cDNA), mouse Pgk promoter, phage T7 gb2 promoter, E. coli Neomycine resistant gene, mouse Pgk PolyA, yeast FRT (flipase recombination target) sites, mouse Avp genomic DNA |
Research application | FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur J Neurosci. 42:2678-2689, 2015. doi: 10.1111/ejn.13061. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior nortification of the use of C57BL/6J-(AVPELuc) mouse. |
Depositor | Sato Honma (Hokkaido University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |