Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC09657  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Clec4a4<tm1.1Ksat>  
Former Common name Clec4a4-/- mice  
H-2 Haplotype No Data  
ES cell line Bruce 4 [B6.Cg-Thy1]  
Background strain C57BL/6JJcl  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to C57BL/6J for 9 generations.  
Strain description Clec4A4 (DCIR2) knockout mice. IRES-EGFP cassette was replaced with exon 1 and exon 2 of the Clec4A4 gene. No GFP expression was detected in the mice.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Clec4a4  
Symbol name C-type lectin domain family 4, member a4  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol Ires2  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Commun., 7, 11273 (2016).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a "MTA for Clec4a4-IRES2EGFP KI transgenic mice for Academic Research Purposes" specified by the DEPOSITOR and Inserm.  
Additional information
 1 MTA for Clec4a4-IRES2EGFP Knock-In Mice for Academic Research Purposes (English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Depositor's present address; Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.  
 2 Genotyping protocol <PCR>  
Depositor Sato, Katsuaki (RIKEN Research Center For Allergy and Immunology)  Sato, Katsuaki 
Strain Status /
(Expected delivery)

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