Strain Data Sheet

RBRC09667

Strain Information

Image
BRC No.RBRC09667
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(CB)-Gt(ROSA)26Sor<tm2.1(CAG-Fam213b)Watk>
Former Common namemPMPGFS KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kikuko Watanabe, Koshien University in 2016. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 and CBA mixed background.
Strain descriptionKnock-in mice for overexpression of mouse Fam213b. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-Fam213b-pA cassette into the mouse Rosa26 locus. A DNA fragment of loxP-CAT-FRT-PGK-neo-FRT-loxP was removed by crossing to B6;CBA-Tg(CAG-Cre)47Imeg
Colony maintenance
ReferencesJ. Biol. Chem., 283, 792-801 (2008). 18006499
J. Biochem., 150, 593-596 (2011). 21926128
Genes. Dev., 5, 1513-1523 (1991). 1653172

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2.1(CAG-Fam213b)Watk>targeted mutation 2.1, Kikuko Watanabe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fam213bfamily with sequence similarity 213, member B6Fam213bCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Ordering Information

Donor DNAbovine growth hormone polyA signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP site, mouse Fam213b cDNA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biochem., 150, 593-596 (2011). J. Biol. Chem., 283, 492-801 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested.
DepositorKikuko Watanabe (Kansai Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data