Strain Data Sheet

RBRC09668

Strain Information

Image
BRC No.RBRC09668
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ndrg2<tm1.1Hori>
Former Common nameNdrg2 Knockout mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Osamu Hori, Kanazawa University. ES cell line in 129Sv/J background was used. Mice were backcrossed to C57BL/6 for 8 generations.
Strain descriptionNdrg2 knockout mice. Exon 3 and 4 of the Ndrg2 gene were replaced with a pgk-neo cassette and then it was removed by crossing to CAG-Cre mice.
Colony maintenance
ReferencesJ. Neurochem., 130, 374-387 (2014). 24697507

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ndrg2N-myc downstream regulated gene 214Ndrg2<tm1.1Hori>targeted mutation 1.1, Osamu Hori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Ordering Information

Donor DNAphage P1 loxP site, mouse Ndrg2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 130, 374-387 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
DepositorOsamu Hori (Kanazawa University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data