Strain Data Sheet

RBRC09676

Strain Information

Image
BRC No.RBRC09676
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Camk2a-Crem)I19Nkoji
Former Common nameICER-I-OE
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shogo Endo and Nobuhiko Kojima, Tokyo Metropolitan Institute of Gerontology. The Camk2a promoter was used to drive brain expression of splice variant I of Crem.A DNA construct was injected to fertilized eggs in B6/C3H/F1 background (RBRC09675) or C57BL6/J background (RBRC09676, RBRC09677). Mice were backcrossed to C57BL/6J.
Strain descriptionICER(Inducible cAMP early repressor)-I overexpressing mice under the control of CaMKIIα promoter. No overt change in brain anatomy and general behavior. Impairment in consolidation of fear memory. Attenuation of amygdala kindling.
Colony maintenance
ReferencesJ. Neurosci., 28, 6459-6472 (2008). 18562617

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CremcAMP responsive element modulatorUNCremmouse Camk2a promoter

Ordering Information

Donor DNAM Musculus CaMKIIα promoter, adenovirus tripartite leader sequence +14〜+200, Mus musculus IgG variable region(3'-splice sequence), M Musculus ICER-I cDNA, SV40 polyA signal
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 28, 6459-6472 (2008).The RECIPIENT must close MTA with Tokyo Metropolitan Institute of Gerontology.
DepositorShogo Endo (Tokyo Metropolitan Institute of Gerontology)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data