Strain Data Sheet

RBRC09680

Strain Information

Image
BRC No.RBRC09680
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Crem<tm1Endo>
Former Common nameICER-flox, C57BL/6-Crem<tm1.2Nkoji>
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shogo Endo and Nobuhiko Kojima, Tokyo Metropolitan Institute of Gerontology. ES cell line (MS12) derived from C57BL/6NSlc was used. Mice were backcrossed to C57BL/6J.
Strain descriptionFloxed CREM mice. P2 exon was replaced with a floxed fragment containing P2 exon and FRT-flanked neo cassette. The neo cassette were then removed by microinjection of a flippase plasmid to fertilized eggs.
Colony maintenance
ReferencesJ. Neurosci., 28, 6459-6472 (2008). 18562617

Health Report

Examination Date / Room / Rack2022/05/31Room:4-BRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CremcAMP responsive element modulator18Crem<tm1Endo>targeted mutation 1, Shogo Endo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Ordering Information

Donor DNAEnterobacteria phage P1 loxP sites, Saccharomyces cerevisiae frt site, mouse Crem genomic DNA
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 28, 6459-6472 (2008).The RECIPIENT must close MTA with Tokyo Metropolitan Institute of Gerontology.
DepositorShogo Endo (Tokyo Metropolitan Institute of Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data