Strain Information | |
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Image | |
BRC No. | RBRC09680 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Crem<tm1Endo> |
Former Common name | ICER-flox, C57BL/6-Crem<tm1.2Nkoji> |
H-2 Haplotype | |
ES Cell line | MS12 [C57BL/6] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Shogo Endo and Nobuhiko Kojima, Tokyo Metropolitan Institute of Gerontology. ES cell line (MS12) derived from C57BL/6NSlc was used. Mice were backcrossed to C57BL/6J. |
Strain description | Floxed CREM mice. P2 exon was replaced with a floxed fragment containing P2 exon and FRT-flanked neo cassette. The neo cassette were then removed by microinjection of a flippase plasmid to fertilized eggs. |
Colony maintenance | |
References | J. Neurosci., 28, 6459-6472 (2008). 18562617 |
Health Report | |
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Examination Date / Room / Rack | 2022/05/31Room:4-BRack:J |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site18Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP |
Ordering Information | |
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Donor DNA | Enterobacteria phage P1 loxP sites, Saccharomyces cerevisiae frt site, mouse Crem genomic DNA |
Research application | Cre/loxP system FLP/frt system Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 28, 6459-6472 (2008).The RECIPIENT must close MTA with Tokyo Metropolitan Institute of Gerontology. |
Depositor | Shogo Endo (Tokyo Metropolitan Institute of Gerontology) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |