Strain Information | |
---|---|
Image | |
BRC No. | RBRC09690 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Gpr65<Tn(sb-lacZ,GFP)T1.88Jtak>/Sati |
Former Common name | Gpr65gt/gt |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Junji Takeda, Osaka University in 2003. Backcrossed to C57BL/6 for 9 generations by Satoshi Ishii, Akita University Graduate School of Medicine. |
Strain description | Gene trap mice of the Gpr65 gene with a sleeping beauty transposable element. Homozygous mice are viable and fertile, but they show abnormal macrophage physiology and osteoclastic activity. |
Colony maintenance | |
References | J. Neurochem., 129, 683-695 (2014). 24447140 J. Immunol., 182, 3243-3251 (2009). 19234222 FASEB J., 28, 871-879 (2014). 24221084 Mol. Cell Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gpr65G-protein coupled receptor 6512Gpr65<Tn(sb-lacZ,GFP)T1.88Jtak>transposon insertion T1.88, Junji Takeda Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BCL2Splice acceptor (human bcl-2)12BCL2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)12CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hprtmouse Hprt splice donor (SD)12Hprt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP |
Ordering Information | |
---|---|
Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Phage P1 loxP sites, Salmon Sleeping Beauty transposase, Human bc1-2 splice acceptor (SA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, Rabbit beta-globin adenylation signal sequence, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, Mouse Hprt splice donor (SD), mRNA instability (human granulocyte-macrophagecolony-stimulating factor cDNA) |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 23, 9189-9207 (2003). FASEB J., 28, 871-879 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Satoshi Ishii (Akita University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |