Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC09690  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Gpr65<Tn(sb-lacZ,GFP)T1.88Jtak>/Sati  
Former Common name Gpr65gt/gt  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Junji Takeda, Osaka University in 2003. Backcrossed to C57BL/6 for 9 generations by Satoshi Ishii, Akita University Graduate School of Medicine.  
Strain description Gene trap mice of the Gpr65 gene with a sleeping beauty transposable element. Homozygous mice are viable and fertile, but they show abnormal macrophage physiology and osteoclastic activity.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Gpr65  
Symbol name G-protein coupled receptor 65  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 2 Symbol Ires  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 3 Symbol SA  
Symbol name Splice acceptor (human bcl-2)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 4 Symbol SD  
Symbol name mouse Hprt splice donor (SD)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 5 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 6 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell Biol., 23, 9189-9207 (2003). FASEB J., 28, 871-879 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Ishii, Satoshi (Akita university)  Ishii, Satoshi 
Strain Status /
Availability
(Expected delivery)

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