Strain Data Sheet

RBRC09703

Strain Information

Image
BRC No.RBRC09703
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mta2<tm1Nkcc>
Former Common nameMta2 KO
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Soichi Takiguchi, National Hospital Organization Kyushu Cancer Center, in collaboration with Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2002. E14TG2aIV ES cell line (129P2/OlaHsd) was used. C57BL/6J congenic strain (backcrossed to C57BL/6J for 12 generations).
Strain descriptionMta2 (metastasis-associated gene family, member 2) knockout mice. A genomic fragment corresponding from exon 8 to exon 12 of the Mta2 gene was replaced with a Neo resistance gene. The rate of homozygous mutants born from hetero x hetero crossing is much lower than what is expected from the Mendel’s law of inheritance.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mta2metastasis-associated gene family, member 219Mta2<tm1Nkcc>targeted mutation 1, Soichi Takiguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19mouse RNA polymerase II promoter (PolII promoter)

Ordering Information

Donor DNAmouse Polr2a promoter, E. coli neo, human hypoxanthine phosphoribosyl transferase 1 polyA signal, mouse Mta2 genomic DNA
Research applicationCancer Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use or publication with the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. However, it may not be a collaboration if the research interest is out of field of the DEPOSITOR.
DepositorSoichi Takiguchi (National Hospital Organization Kyushu Cancer Center)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data