Strain Information | |
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Image | |
BRC No. | RBRC09710 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Slc25a24<tm1Sinos> |
Former Common name | Slc25a24-KO, Slc25a24 knockout |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Satoshi Inoue, Saitama Medical University (2015). Bruce 4 ES cells were used. The mice were backcrossed to C57BL/6J. |
Strain description | Slc25a24 [solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 24] knockout mice. Exon 2 was replaced with a SV40 polyA-Neo cassette. Slc25a24-KO mice have significantly lower body weights and white adipose tissue weights. Adipocyte differentiation is inhibited in Slc25a24-KO adipose tissues. |
Colony maintenance | |
References | J. Clin. Endocrinol. Metab., 100, E655-E663 (2015). 25599384 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Slc25a24solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 243Slc25a24<tm1Sinos>targeted mutation 1, Satoshi Inoue Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal3 |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Simian virus 40 SV40 polyA, mouse Slc25a24 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use the BIOLOGICAL RESOURSE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Satoshi Inoue (Saitama Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |