Strain Data Sheet

RBRC09710

Strain Information

Image
BRC No.RBRC09710
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Slc25a24<tm1Sinos>
Former Common nameSlc25a24-KO, Slc25a24 knockout
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Satoshi Inoue, Saitama Medical University (2015). Bruce 4 ES cells were used. The mice were backcrossed to C57BL/6J.
Strain descriptionSlc25a24 [solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 24] knockout mice. Exon 2 was replaced with a SV40 polyA-Neo cassette. Slc25a24-KO mice have significantly lower body weights and white adipose tissue weights. Adipocyte differentiation is inhibited in Slc25a24-KO adipose tissues.
Colony maintenance
ReferencesJ. Clin. Endocrinol. Metab., 100, E655-E663 (2015). 25599384

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc25a24solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 243Slc25a24<tm1Sinos>targeted mutation 1, Satoshi Inoue

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal3

Ordering Information

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Simian virus 40 SV40 polyA, mouse Slc25a24 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use the BIOLOGICAL RESOURSE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorSatoshi Inoue (Saitama Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data