Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC09714  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>  
Former Common name Chx10-IRES-CreERT2 KI  
H-2 Haplotype No Data  
ES cell line JM8A3 [C57BL/6N-A]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. This knock-in mice were mated to mice in B6.C3H background and then mated to mice in C57BL/6J background.  
Strain description IRES-CreERT2 knock-in mice. An IRES-CreERT2 cassette was placed just downstream of stop codon of the Vsx2 (visual system homeobox 2, Chx10) gene. A Neo gene cassette was removed by a CAG-Cre mouse line. IRES-CreERT2 can be removed by a Flp recombinase.  
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol ERT2  
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 3 Symbol Vsx2  
Symbol name visual system homeobox 2  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 4 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 12  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
FLP/frt system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University)  Furukawa, Takahisa 
Strain Status /
Availability
(Expected delivery)

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