Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC09728  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg(C)-Hist1h2aa/Hist1h2ba<tm1Sis>  
Former Common name Th2a- Th2b-  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Shunsuke Fujii, RIKEN in 2014. R1 ES cell line [(129X1/SvJ x 129S1)F1] was used. After BALB/cA congenic line had been established, this line was backcrossed to C57BL/6J for 5 generations. BALB/cA congenic line is available at RBRC09729.  
Strain description A 3.6 kb region, which covers both Hist1h2aa and Hist1h2ba, was replaced with a neo cassette via homologous recombination. It is expected that male homozygotes are sterile and female homozygotes have low fertility.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Hist1  
Symbol name histone cluster 1  
Chromosome 13  
Common name Th2a- Th2b-  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 13  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cell Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Stem Cell., 14, 217-227 (2014). Development, 142, 1287-1292 (2015).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Ishii, Shunsuke (RIKEN)  
Strain Status /
Availability
(Expected delivery)

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