Strain Data Sheet

RBRC09728

Strain Information

Image
BRC No.RBRC09728
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg(C)-Hist1h2aa/Hist1h2ba<tm1Sis>
Former Common nameTh2a- Th2b-
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Shunsuke Fujii, RIKEN in 2014. R1 ES cell line [(129X1/SvJ x 129S1)F1] was used. After BALB/cA congenic line had been established, this line was backcrossed to C57BL/6J for 5 generations. BALB/cA congenic line is available at RBRC09729.
Strain descriptionA 3.6 kb region, which covers both Hist1h2aa and Hist1h2ba, was replaced with a neo cassette via homologous recombination. It is expected that male homozygotes are sterile and female homozygotes have low fertility.
Colony maintenance
ReferencesDevelopment, 142, 1287-1292 (2015). 25742800
Cell Stem Cell., 14, 217-227 (2014). 24506885

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hist1histone cluster 113Hist1<tm1Sis>targeted mutation 1, Shunsuke IshiiTh2a- Th2b-

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), E coli Neomycin resistance gene, mouse Phosphoglycerate kinase polyA signal, mouse Hist1h2aa/Hist1h2ba genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Stem Cell., 14, 217-227 (2014). Development, 142, 1287-1292 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN BRC and must obtain a prior written consent from the RIKEN BRC RECIPIENT must contact the RIKEN BRC in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
DepositorShunsuke Ishii (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data