Strain Information | |
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Image | |
BRC No. | RBRC09728 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg(C)-Hist1h2aa/Hist1h2ba<tm1Sis> |
Former Common name | Th2a- Th2b- |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Shunsuke Fujii, RIKEN in 2014. R1 ES cell line [(129X1/SvJ x 129S1)F1] was used. After BALB/cA congenic line had been established, this line was backcrossed to C57BL/6J for 5 generations. BALB/cA congenic line is available at RBRC09729. |
Strain description | A 3.6 kb region, which covers both Hist1h2aa and Hist1h2ba, was replaced with a neo cassette via homologous recombination. It is expected that male homozygotes are sterile and female homozygotes have low fertility. |
Colony maintenance | |
References | Development, 142, 1287-1292 (2015). 25742800 Cell Stem Cell., 14, 217-227 (2014). 24506885 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse Phosphoglycerate kinase promoter (PGK promoter), E coli Neomycin resistance gene, mouse Phosphoglycerate kinase polyA signal, mouse Hist1h2aa/Hist1h2ba genomic DNA |
Research application | Cell Biology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Stem Cell., 14, 217-227 (2014). Development, 142, 1287-1292 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN BRC and must obtain a prior written consent from the RIKEN BRC RECIPIENT must contact the RIKEN BRC in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE. |
Depositor | Shunsuke Ishii (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |