Strain Information | |
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Image | |
BRC No. | RBRC09731 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR.Cg-Braf<tm1.1Tumg> |
Former Common name | Braf Q241R/+ ICR (Braf Q241R knock-in mice; ICR background) |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. RBRC09730 was crossed with CAG-Cre and then backcrossed to ICR for 11 generations. |
Strain description | Braf-mutated protein (BrafQ241R) knock-in mice in ICR genetic background. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. Conditional BrafQ241R knock-in mice are available as RBRC09730. |
Colony maintenance | |
References | Human Molecular Genetics, 24, 7349-7360 (2015). 26472072 Human Molecular Genetics, 23, 6553-6566 (2014). 25035421 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP site, mouse Braf genomic DNA |
Research application | Cancer Research Cell Biology Research Cre/loxP system Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Human Molecular Genetics, 23, 6553-6566 (2014). Human Molecular Genetics, 24, 7349-7360 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Yoko Aoki (Tohoku University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |