Strain Information | |
---|---|
Image | |
BRC No. | RBRC09743 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C3.129P2-Ambp<tm1Kkm> |
Former Common name | Bikunin knockout mouse, UTI knockout mice, ITILC knockout mouse |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C3H/HeSlc |
Appearance | |
Strain development | Developed by Lisheng Zhuo and Koji Kimata, Aichi Medical University in 2000. E14 ES cell was used. C3He congenic line. Other congenic lines in C57BL/6NCrSlc (RBRC09742), DBA1/JJms (RBRC09744) or BALB/cCrSlc (RBRC09745) are also available. |
Strain description | Ambp (alpha 1 microglobulin/bikunin)knockout mice. A neo cassette replaced exons coding the Bikunin protein, but unchanged exons coding the alpha 1 microglobulin protein. Homozygous females are infertile. |
Colony maintenance | |
References | Int. Arch. Allergy Imm., 153, 223-233 (2010). 20484920 Am. J. Physiol. Gastrointest. Liver. Physiol., 305, G797-806 (2013). 24113769 J. Biol. Chem., 276, 7693-7696 (2001). 11145954 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 Late and early polyA signal4 |
Ordering Information | |
---|---|
Donor DNA | HSV thymidine kinase promoter, E coli Neomycin resistance gene, HSV thymidine kinase polyA signal, SV40 Late and early polyA signal (a 139 bp fragment between HpaI and BamHI sites in pMAMneo-S vector(Clontech), mouse Ambp genomic DNA |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Koji Kimata (Aichi Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |