Strain Information | |
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Image | |
BRC No. | RBRC09768 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Gt(ROSA)26Sor<tm2(CAG-HIST1H2BJ)Shna>/24 |
Former Common name | flagH2B #24, #19 |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Shinichi Nakagawa, RIKEN. C57BL/6 background. TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Mice were backcrossed to C57BL/6 for 6 generations. CDB1193K. |
Strain description | Knock-in mice for Cre-dependent overexpression of human histone cluster 1, H2bj (HIST1H2BJ). Targeted insertion of CAG-loxP-pgk-neo-3xpA-loxP-Flag-HIST1H2BJ-pA cassette into the mouse Rosa26 locus. Flag-tag was fused to HIST1H2BJ N-terminally. A strain deleting pgk-neo-3xpA by Cre is available as RBRC09769. |
Colony maintenance | |
References | Sci. Rep., 8(1):1143 (2018). 29348483 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(CAG-HIST1H2BJ)Shna>targeted mutation 2, Shinichi Nakagawa Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)6FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HIST1H2BJhistone cluster 1, H2bj (human)6HIST1H2BJCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Enterobacteria phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 polyA, FLAG tag sequence (synthetic), human histone cluster 1, H2bj (HIST1H2BJ), bovine growth hormone polyA signal, mouse ROSA26 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep., 8(1):1143 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Shinichi Nakagawa (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |