Strain Data Sheet

RBRC09770

Strain Information

Image
BRC No.RBRC09770
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-6330403k07Rik<tm1.1Shna>
Former Common name6330403k07Rik(LacZ)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Nakagawa, RIKEN. C57BL/6 background. TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Mice were backcrossed to C57BL/6 for 5 generations. CDB1164K.
Strain description6330403k07Rik mutant mice. A part of exon 1 was replaced by lacZ-FRT-neo-FRT. The Neo cassette was removed by crossing 129S4/SvJaeSor-Gt(ROSA)26Sor<tm1(FLP1)Dym>/J.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
6330403k07RikRIKEN cDNA 6330403K07 gene116330403k07Rik<tm1.1(lacZ)Shna>targeted mutation 1.1, Shinichi Nakagawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA11HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pgk1mouse PGK polyA signal11Pgk1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence11lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

Donor DNAP1ファージ Lox71, P1ファージ loxP, 大腸菌 LacZ, 酵母菌 frt, マウス Pgk polyA, ウサギ Rabbit beta-globbin polyA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShinichi Nakagawa (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data