Strain Data Sheet

RBRC09771

Strain Information

Image
BRC No.RBRC09771
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129B6-Dgcr14<tm1c(KOMP)Nbioc>
Former Common nameDgcr14 flox
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Ichiro Takada, Nihon University of School of Medicine. Mixed genetic background of 129S4 and C57BL/6.
Strain descriptionFloxed mice for Dgcr14 (DiGeorge syndrome critical region gene 14). loxP sites are placed at intron 3 and 8. Targeting vector (PG00129_Y_4_A10) was obtained from the Wellcome Trust Sanger Institute. RENKA ES cells derived from C57BL/6N were used. Mice were further crossed with 129S4/Sv.JaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J to remove lacZ and neo. Dgcr14 knockout mice are available as RBRC09772.
Colony maintenance
ReferencesJ. Biol. Chem., 102342. doi: 10.1016/j.jbc.2022.102342.(2022). 35933014
Sci. Rep., 13(1):12355 (2023). 37524814

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dgcr14DiGeorge syndrome critical region gene 1416Dgcr14<tm1c(KOMP)Nbioc>targeted mutation 1c, Makoto Makishima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Dgcr14 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorMakoto Makishima (Nihon University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data