Strain Data Sheet
RBRC09776
Strain Information | |
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| Image | |
| BRC No. | RBRC09776 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Efcab3<tm1a(KOMP)Osb>/40 |
| Former Common name | Efcab3#40 |
| H-2 Haplotype | |
| ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Haruhiko Miyata and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2012. C57BL/6N genetic background. |
| Strain description | Floxed Efcab3 line. A pair of loxP flanks Exon 6. |
| Colony maintenance | |
| References | Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Efcab3EF-hand calcium binding domain 311Efcab3<tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)11En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)11 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Apolyadenylic acid additional signal11poly A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Apolyadenylic acid additional signal11poly A |
Ordering Information | |
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| Donor DNA | [chicken beta-actin promoter, rabbit beta-globin polyadenylation signal,CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter], Yeast FRT sites, mouse En2 splice acceptor (En2 intron2/exon 3 splice acceptor), EMCV Internal ribosomal entry site (IRES), E. coli. lacZ, SV40 polyadenylation site, Phage P1 loxP sites, human Beta-actin promoter, E. coli. Neomycin phosphotransferase gene (neoR) , mouse Efcab3 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |