Strain Information | |
---|---|
Image | |
BRC No. | RBRC09796 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ano6<tm1.1Naga> |
Former Common name | TMEM16F Flox |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Shigekazu Ngata, Jun Suzuki and Toshihiro Fujii at Osaka University in 2013. Bruce 4 ES cell line was used. Neomycin resistance gene was removed by crossing CAG-FLPe mice. A pair of loxP are flanking exon 2. |
Strain description | Floxed mice for Ano6(anoctamin 6, Tmem16f). |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). 26417084 J. Biol. Chem., 288, 13305-13316 (2013). 23532839 Nature, 468, 834-838 (2010). 21107324 |
Health Report | |
---|---|
Examination Date / Room / Rack | 2024/02/19Room:4-ARack:E 2023/11/20Room:4-ARack:E 2023/08/21Room:4-ARack:E 2023/05/22Room:4-ARack:E 2023/02/20Room:4-ARack:E 2023/02/20Room:4-ARack:E 2022/11/21Room:4-ARack:E 2022/08/22Room:4-ARack:E 2022/05/23Room:4-ARack:E |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP |
Ordering Information | |
---|---|
Donor DNA | Phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ano6 genome DNA |
Research application | Cre/loxP system FLP/frt system Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). J. Biol. Chem., 288, 13305-13316 (2013).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Osaka University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |