Strain Data Sheet

RBRC09805

Strain Information

Image
BRC No.RBRC09805
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(Myh6-HK2)BChuan
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takashi Iwamoto, Chubu University in 2015. B6D2F1 fertilized eggs were used for microinjection. Mice were backcrossed to C57BL/6J three times.
Strain descriptionTransgenic mouse line overexpressing human hexokinase 2 (HK2) under the control of rat Myh6 (myosin, heavy polypeptide) promoter. Hexokinase 2, which phosphorylates glucose to form G6P, is a key enzyme in glucose metabolism. A growing body of evidence has shown that HK2 is involved in cardioprotection. These transgenic mice overexpress human HK2 in the heart muscle and have no obvious abnormality. It was reported that cardiac overexpression of mouse HK2 attenuated hypertrophy by increasing pentose phosphate pathway. Two sublines are available (A, RBRC09804; B, RBRC09805)
Colony maintenance
ReferencesJ. Am. Heart Assoc. 2013 Nov 4;2(6):e000355. 24190878

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HK2Hexokinase 2 (human)UNHK2Rat Myh6 promoter

Ordering Information

Donor DNAHomo sapiens Growth hormone polyA signal, Mouse alpha-myosin heavy chain promoter, Homo sapiens Hexokinase 2
Research applicationCardiovascular Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakashi Iwamoto (Chubu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data