Strain Data Sheet
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Data update: Jul 19, 2019
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RBRC No. RBRC09860  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.B6D2-Tg(Thy1-FUS<*>deltaNLS)1Dit  
Former Common name deltaNLF-FUStg  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development A human FUS cDNA without a nuclear localization signal was inserted into the pTSC21k vector, which encodes the 8.1 kb murine Thy1.2 expression cassette. This construct was Not I digested to remove vector sequences, co-injected into the pronucleus of fertilized eggs from Slc:BDF1 strain mice. Founders were bred and backcrossed with C57Bl6/J mice to establish transgenic lines.  
Strain description A transgenic (tg) mouse overexpressing exogenous FUS without a nuclear localization signal (ΔNLS-FUS) develop progressive spastic motor deficits and neuronal loss in the motor cortex. The ΔNLS-FUS protein was restricted to the cytoplasm and formed ubiquitin/p62-positive aggregates. Endogenous FUS expression, nuclear localization, and splicing activity were not altered, indicating that mislocated FUS is sufficient for proteinopathy. RNA-sequence analysis revealed specific transcriptome alterations, including genes regulating dynein-associated molecules and endoplasmic reticulum stress.  
Colony maintenance Founders were bred and backcrossed with C57Bl6/J mice to establish transgenic lines.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name No Data  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Brain, 139, 2380-2394 (2016).  
Additional information
 1 No Data  
Depositor Ito, Daisuke (Keio University School of Medicine)  Ito, Daisuke 
Strain Status /
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