Strain Data Sheet

RBRC09861

Strain Information

Image
BRC No.RBRC09861
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Calcr<tm1c(KOMP)Sfuk>
Former Common nameCalcr-floxed mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by So-ichiro Fukada, Osaka University. EGR-G01(129S2×C57BL/6NCr)ES cells were used. Mice were backcrossed to C57BL/6 for more than 4 generations after crossing to a CAG-Flpe line.
Strain descriptionCalcr (calcitonin receptor) knockout mice.
Colony maintenance
ReferencesCell Reports, 13, 302-314 (2015). 26440893

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Calcrcalcitonin receptor6Calcr<tm1c(KOMP)Sfuk>targeted mutation 1c

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Ordering Information

Donor DNAyeast FRT (flippase recombination target) site, phage P1 loxP sites, mouse Calcr genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Reports, 13, 302-314 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. 　In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorSoichirou Fukada (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data