Strain Data Sheet

RBRC09894

Strain Information

Image
BRC No.RBRC09894
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Has2<tm1.1Nita>
Former Common nameHas2 conditional knockout
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naoki Itano, Kyoto Sangyo University in 2006. An ES cell line derived from 129/SvEvTac was used. This line was crossed with a CAG-FLPe line to remove FRT-flanked neo cassette, and then backcrossed to C57BL/6 for 7 generations.
Strain descriptionFloxed Has2 mice. Homozygous null mutants are embyonically lethal.
Colony maintenance
ReferencesCancer Res., 70(18), 7073-7083(2010). 20823158

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Has2hyaluronan synthase 215Has2<tm1.1Nita>targeted mutation 1.1, Naoki Itano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Ordering Information

Donor DNAPhage P1 loxP sites, yeast FRT recombinase target site, mouse Has2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNaoki Itano (Kyoto Sangyo University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data