Strain Information | |
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Image | |
BRC No. | RBRC09894 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Has2<tm1.1Nita> |
Former Common name | Has2 conditional knockout |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Naoki Itano, Kyoto Sangyo University in 2006. An ES cell line derived from 129/SvEvTac was used. This line was crossed with a CAG-FLPe line to remove FRT-flanked neo cassette, and then backcrossed to C57BL/6 for 7 generations. |
Strain description | Floxed Has2 mice. Homozygous null mutants are embyonically lethal. |
Colony maintenance | |
References | Cancer Res., 70(18), 7073-7083(2010). 20823158 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, yeast FRT recombinase target site, mouse Has2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Naoki Itano (Kyoto Sangyo University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |