Strain Information | |
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Image | |
BRC No. | RBRC09911 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6N-Sik3<em2Iiis> |
Former Common name | Sik3<Flag> |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NJcl |
Appearance | |
Strain development | Developed by Hiromasa Funato and Masashi Yanagisawa, University of Tsukuba. C57BL/6N background. |
Strain description | Mouse line harboring a Flag-tag inserted into the N terminus of SIK3 (wildtype) by the CRISPR/Cas9 technology. This line exhibits normal sleep and wakefulness and breeds well. |
Colony maintenance | |
References | Nature, 539, 378-383 (2016). 27806374 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sik3SIK family kinase 39Sik3<em2Iiis>endonuclease-mediated mutation 2, Masashi Yanagisawa and Hiromasa Funato Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)9FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence9HA |
Ordering Information | |
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Donor DNA | mouse Sik3 genomic DNA, Synthetic FLAG tag, Human Influenza HA tag (Hemagglutinin gene) |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Drs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Mar 2019 |
BRC mice in Publications |
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No Data |