Strain Information | |
---|---|
Image | |
BRC No. | RBRC09912 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6N-Sik3<em3Iiis> |
Former Common name | Sik3<Flag,Slp> |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NJcl |
Appearance | |
Strain development | Developed by Hiromasa Funato and Masashi Yanagisawa, University of Tsukuba. C57BL/6N background. Flag-tag was inserted into RBRC09910 by the CRISPR/Cas9 technology. |
Strain description | Mouse line harboring a Flag-tag inserted into the N terminus of mutated SIK3 found in the Sleepy (Slp) mutant. Heterozygous males and females are prone to be poor breeders. |
Colony maintenance | |
References | Nature, 539, 378-383 (2016). 27806374 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sik3SIK family kinase 39Sik3<em3Iiis>endonuclease-mediated mutation 3, Masashi Yanagisawa and Hiromasa Funato Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)9FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence9HA |
Ordering Information | |
---|---|
Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], mouse Sik3 genomic DNA, Synthetic FLAG tag, Human Influenza HA tag (Hemagglutinin gene), yeast FRT (flipase recombination target) site |
Research application | FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Drs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Mouse of the Month Mar 2019 Necessary documents for ordering: |
BRC mice in Publications |
---|
No Data |