Strain Data Sheet

RBRC09914

Strain Information

Image
BRC No.RBRC09914
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Drd2<tm1.1Mmsh>
Former Common nameDrd2 floxed, B6-Drd2 floxed, fDrd2
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Noboru Mizushima, The University of Tokyo. RENKA ES cells derived from C57BL/6N was used and mice were maintained by brother-sister mating. A neo cassette was removed by crossing with B6-Tg(CAG-FLPe)36.
Strain descriptionFloxed Drd2 mice. Exon 2 of Drd2 is flanked by loxP sites. Conditional Drd2 deficient mice can be generated by crossing with cell type-specific Cre driver mice.
Colony maintenance
ReferencesSci. Rep., 4:3976 (2014). 24496082

Health Report

Examination Date / Room / Rack2024/02/26Room:4-CRack:D
2023/11/27Room:4-CRack:D
2023/08/28Room:4-CRack:D
2023/05/29Room:4-CRack:D
2023/02/27Room:4-CRack:D
2022/11/29Room:4-CRack:D
2022/08/29Room:4-CRack:D
2022/05/31Room:4-CRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Drd2dopamine receptor D29Drd2<tm1.1Mmsh>targeted mutation 1.1, Masayoshi Mishina

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Drd2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR during first TWO years after deposition by the DEPOSITOR to the RIKEN BRC. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayoshi Mishina (Ritsumeikan University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data