Strain Data Sheet

RBRC09916

Strain Information

Image
BRC No.RBRC09916
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Bclaf1<em2Nuc>
Former Common nameBtf KO No.28(hetero)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Tokuko Haraguchi, Graduate School of Frontier Biosciences, Osaka University. With the CRISPR/Cas9, 1bp insertion was introduced at exon 4 of the Bclaf1 gene. C57BL/6N genetic background.
Strain descriptionBclaf1 (BCL2-associated transcription factor 1) mutant mice. Homozygous mutatns are posnatally lethal.Bclaf1<em2Nuc>: 1bp insertion at exon 4.GTCGAGATT(T)ACAGGAATAATAGAGGA
Colony maintenance
ReferencesEur. J. Biochem., 271, 1035-1045 (2004). 15009215

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bclaf1BCL2-associated transcription factor 110Bclaf1<em2Nuc>endonuclease-mediated mutation 2, Tokuko Haraguchi

Ordering Information

Donor DNAmouse Btf(Bcl-associated transcription factor), pX330 vector (Streptococcus pyogenes Cas9*, Escherichia coli ampicillin resistant gene, synthetic FLAG tag)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research applicationMouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from　Dr. Tokuko Ｈａｒａｇｕｃｈｉ. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University.
DepositorYasushi Hiraoka (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data