Strain Information | |
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Image | |
BRC No. | RBRC09916 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6N-Bclaf1<em2Nuc> |
Former Common name | Btf KO No.28(hetero) |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrSlc |
Appearance | |
Strain development | Developed by Tokuko Haraguchi, Graduate School of Frontier Biosciences, Osaka University. With the CRISPR/Cas9, 1bp insertion was introduced at exon 4 of the Bclaf1 gene. C57BL/6N genetic background. |
Strain description | Bclaf1 (BCL2-associated transcription factor 1) mutant mice. Homozygous mutatns are posnatally lethal.Bclaf1<em2Nuc>: 1bp insertion at exon 4.GTCGAGATT(T)ACAGGAATAATAGAGGA |
Colony maintenance | |
References | Eur. J. Biochem., 271, 1035-1045 (2004). 15009215 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Bclaf1BCL2-associated transcription factor 110Bclaf1<em2Nuc>endonuclease-mediated mutation 2, Tokuko Haraguchi |
Ordering Information | |
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Donor DNA | mouse Btf(Bcl-associated transcription factor), pX330 vector (Streptococcus pyogenes Cas9*, Escherichia coli ampicillin resistant gene, synthetic FLAG tag)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Tokuko Haraguchi. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University. |
Depositor | Yasushi Hiraoka (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |