Strain Data Sheet

RBRC09923

Strain Information

Image
BRC No.RBRC09923
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Zeb1<tm3.1Yhi>
Former Common namezeb1 flox mouse, zfhx1a flox mouse
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Yujiro Higashi, Institute for Developmental Research in 2012. EGR-G101 ES cell line was used and this strain was maintained in C57BL/6N genetic background.
Strain descriptionFloxed Zeb1(Zfhx1a, zinc finger E-box binding homeobox 1)mice. Floxed Zeb2(Zfhx1b, SIP1)mice are also available as RBRC01924.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zeb1zinc finger E-box binding homeobox 118Zeb1<tm3.1Yhi>targeted mutation 3.1, Yujiro Higashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Ordering Information

Donor DNABacteriophage P1 loxP sites, Yeast FRT site, mouse Zfhx1a genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYujiro Higashi (Aichi Prefectural Colony)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data